Jul 02, 2018 preparative routine purification hitrap hp 1 ml and 5 ml iex columns hitrap sp hp and hitrap q hp columns are for routine high resolution, smallscale preparative protein purification. Hitrap chelating hp hitrap chelating hp is one of a range of prepacked 1 ml and 5 ml columns for metal chelate affinity chromatography. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml prepacked columns instructions for use hitrap sp hp and hitrap q hp are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample cleanup of charged biomolecules. Sp sepharose ff average particle size 90 um is usually used in early capture step, whereas sp sepharose hp average particle size 34 um could be used in later polishing step. These cookies are necessary for the website to function and cannot be switched off in our systems. The column is delivered with a stopper on the inlet and a snapoff end on the outlet. Data file 11002576 ag ion exchange chromatography capto s. Po box 507 2999 north wayne st angola, indiana 46703 usa. Ion exchange chromatography principles and methods. Chromatography columns separate molecules within mixtures during gas or liquid chromatography.
We have 5 atlas copco ga22 manuals available for free pdf download. We feature over 1,000 different product lines, many of which are in stock and ready for immediate shipment. How do i connect my wpsenabled wireless router to my product. The sample was applied to a qsepharose hp previously equilibrated according to the manufacturer manual. Hitrap sp ff, hitrap cm ff, hitrap q ff, hitrap deae ff, and hitrap anx. Manual cytiva hitrap deae ffcm ffq ff sp ffanx ff manual. Cytiva formerly ge healthcare life sciences hitrap sp. Recombinant vp1 was purified by hitrap hp column, and sdspage revealed a single band 34 kda, lane 5, close to its theoretical molecular weight 33.
A hitrap q ff 1 ml, b hitrap q ff 5 ml, c hiprep q ff 1610 20 ml. Pdf q sepharose high performance and sp sepharose high. I see the message unable to connect to network scanner. Hitrap sp ff is prepacked with sp sepharose fast flow, and is a strong cation exchanger for smallscale protein purifications as well as. Cytiva formerly ge healthcare life sciences hitrap sp sepharose fast flow iex columns use for smallscale protein purifications cytiva life sciences hitrap sp sepharose fast flow iex columns are strong cation exchanger columns for rapid separations either alone or connected in series. Hitrap capto s, 1 ml hitrap sp xl, 1 ml hitrap sp ff, 1 ml sample. Rinse with at least 2 cv of distilled water until the uvbaseline and eluent ph are stable. Google has many special features to help you find exactly what youre looking for. The industry standard for ion exchange chromatography. Hitrap columns hitrap sp ff, hitrap cm ff, hitrap q ff, hitrap deae ff, and hitrap anx ff high sub are small, affordable, and easytouse prepacked 1 ml or 5 ml iex columns.
If further scaleup is necessary bulk media packages of nprotein a sepharose 4 fast flow are available. Cm sepharosetm fast flow, deae sepharose fast flow, q sepharose fast flow and sp sepharose fast flow are ion exchange chromatography media with excellent flow properties and high capacity for biomolecules. This instruction manual includes product specifications, supplementary installation information, reference materials, custom setup information, maintenance procedures, and replacement part details for the dvc6200f digital valve. Complete, easytofollow instructions are included for fast startup and method optimization. Pdf hiprep sp ff 1610, hiprep cm ff 1610, hiprep q. The instructions that follow are based upon packing sepharose fast flow ion exchange media in the recommended xk 1620 column. Hitrap cm sepharose ff cytiva, formerly ge healthcare life. Hitrapconvenient protein purification fisher scientific. Cm sepharose fast flow deae sepharose fast flow q sepharose. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. For applications where extremely low leakage during purification is critical. Vestil manufacturing corporation is an industry leader in the manufacturing and distributing of industrial material handling equipment.
Human serum, conditions selected to remove igg fraction in pool 1 hitrap rprotein a ff. Manuals or user guides for your hp compaq pro 6305 small form factor pc. Home support scanners fastfoto series epson fastfoto ff680w. Instructions 71715400 ak hitrap desalting columns ge healthcare hitrap desalting hitraptm desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i. Human serum, conditions selected to remove igg fraction in pool 1. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Can one use sp sepharose rather than mono s for protein. Hitrap sp sepharose ff cytiva, formerly ge healthcare life. Wash immediately with 3 cv of start buffer same flow as step 2.
Hp compaq pro 6305 small form factor pc manuals hp. Fisher fieldvue dvc6200f digital valve controller for. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. General instructions for affinity purification using hitrap.
Filled with stationary phase compositions or chemical compounds, the prepacked options reduce the preparing time. Hitrap deae ff, 1 ml and 5 ml hitrap cm ff, 1 ml and 5 ml. Instructions 71700500 ax hitrap affinity columns ge healthcare hitrap chelating hp, 1 ml and 5 ml hitrap chelating hp is a prepacked ready to use, column for preparative affinity chromatography. Hitrap sp xl and hitrap q xl provide fast, reproducible. Predictable scaleuphitrap sp ff is prepacked with sp sepharose fast flow, and is a strong cation exchanger for smallscale protein purifications as well as screening of binding and elutions conditions. Hitrap q hp 1 ml and 5 ml hitrap sp hp 1 ml and 5 ml hitrap q hp and hitrap sp hp are small, prepacked columns made of biocompatible polypropylene. Hitrap sp hp and hitrap q hp provide fast, reproducible, and easy separations in a convenient format. B equilibrate the column, load the sample and begin collecting fractions. Hitrap butyls ff are prepacked butyls sepharose 6 fast flow columns for hydrophobic interaction chromatography hic. Pdf hiprep sp ff 1610, hiprep cm ff 1610, hiprep q ff.
Q sepharose fast flow columns ease of scaleup is a key benefit of working with any sepharose fast flow ion exchanger. Cytiva 17140801 view more versions of this product. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. They are usually only set in response to actions made by you which amount to a request for services, such as setting your privacy preferences, logging in or filling in forms. General instructions for affinity purification using. The sdspage of purified sepharose 6ff column inactivated virus revealed three bands, corresponding to vp0, vp1, and vp3, lane 4. Hitrap sp ff is prepacked with sp sepharose fast flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions. The 1 ml column is often used for method screening to quickly establish optimized binding and elution conditions in packed bed. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml vwr.
Reference manual 0080901004774, rev db july 2020 rosemount 3051 pressure transmitter with foundation fieldbus protocol. Q, sp, deae, and cm sepharose fast flow are based on a robust, 6% highly. Hitrap chelating hp is particularly suitable for the isolation and. Hitrap deae ff is prepacked with deae sepharose fast flow, and is a weak anion exchanger for smallscale protein purifications as well as screening of binding and elutions conditions. Be sure not to exceed flow rates of 1 mlmin 12 dropsec for hitrap 1 ml and 5 mlmin 2 dropsec for hitrap 5 ml at this stage.
Search the worlds information, including webpages, images, videos and more. For use with application for method scouting, group separations, sample concentration and sample cleanup of charged. Sp sepharose high performance is a strong cation exchange medium and q sepharose high performance is a strong anion exchange medium. Q sepharose high performance and sp sepharose high performance. Ion exchange chromatography columns for protein purification. Cation exchange chromatography columns, hitrap sp ff vwr. The columns can be operated with a syringe, peristaltic pump or liquid.
Standing behind every fan we make it our mission to help you with installation, manuals, ceiling fan parts, accessories, and any productrelated questions. Hitrap sp sepharose ff cytiva, formerly ge healthcare. Chromatography columns separate molecules within mixtures during gas or liquid methods. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format. Histrap hp and histrap ff are 1 ml and 5 ml hitrap columns packed with ni sepharose high performance or ni sepharose 6 fast flow, respectively. Avoid cationic detergents with s, sp and cm charged groups. Wash with 4 cv of up to 70% ethanol or 30% isopropanol. Hitrap cm ff is prepacked with cm sepharose fast flow, and is a weak cation exchanger for smallscale protein purifications as well as screening of binding and elutions conditions. Using hitrap q hp and hitrap sp hp prepacked columns is simple. Pdf hiprep sp ff 1610, hiprep cm ff 1610, hiprep q ff 16. Download vestil owners manuals and instruction sheets.
Please read these instructions carefully before using hitrap columns. Hitrap columns, sp sepharose ff, cytiva formerly ge. Dec 21, 2019 hitrap column format is convenient for use with a syringe, a peristaltic pump, or desaltlng chromatography system. Use for smallscale protein purifications cytiva life sciences hitrap sp sepharose fast flow iex columns are strong cation exchanger columns for rapid separations either alone or connected in series. If further scaleup is necessary bulk media packages of nprotein a sepharose 4. All necessary connectors are included for connection to different systems as well as to a laboratory pump and a. Hitrap desalting columns with sephadex g resin ge healthcare life sciences read more the exclusion limit is approximately mr convenient desalting and buffer exchange of sample volumes up to 1. The ion exchange media packed in hitrap ion exchange columns are based on sepharose high performance. The pressure over the packed bed varies depending on a. Hitrap sp ff is prepacked with sp sepharose fast flow, and is a strong cation exchanger for smallscale protein purifications as well as screening of. Instruction book, user manual atlas copco ga22 instruction book 164 pages.
Sample application, washing, and elution can be performed using a syringe with a supplied adapter, a peristaltic pump, or a liquid chromatography system such as akta see chapter 2, manual and. Scope of manual this instruction manual is a supplement to the quick start guide that ships with every instrument. For roto mold spa manuals, see the section further down on this page. Q sepharose high performance sp sepharose high performance. The protein of interest is found in the flow through it elutes in two fractions, the. Find basic information, manuals, and replacement parts for all of our modern, traditional, vintageinspired, and specialty low profile and flush mount fans. Data file 18117722 ad ion exchange chromatography sepharose. Sls scientific laboratory supplies ltd lab supplies. Instructions 71714900 an hitrap ion exchange columns ge healthcare hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml hitrap sp hp and hitrap q hp are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample cleanup of charged biomolecules. Hitrap protein a hp, 1 ml and 5 ml hitrap protein a hp is a prepacked re ady to use, column for preparative purification of monoclonal and polyclonal antibodies. Antithrombin iii from bovine plasma hitrap desalting sample. Data file 18117288 ab ion exhange chromatography q.
Fast, simple, and easy separations are provided by the combination of the prepacked column and a highperformance affinity medium. Hitrap desalting prepacked columns are prepacked, ready to use column for group separation between high and low molecular weight substances. Hitrap sp ff is prepacked with sp sepharose fast flow, and is a strong cation exchanger for smallscale protein purifications as well as screening of binding and elutions conditions. Ion exchange chromatography columns, hitrap sp hp and. Hitrap columns are intended for research use only, and shall not be used in. Check the scanner, network, and connection settings. Hitrap sp xl, 1 ml and 5 ml hitrap q xl, 1 ml and 5 ml.
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